Immunology/Serology Program Modules

Instructions: AE Specimens

(782 ANA Panel)

(783 ANA Panel – Add-On)

ANA Result Codes

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

4. Report ANA screen results as Antinuclear Antibody.

5. Report individual antibodies or Florescence Pattern in the appropriate fields.  Leave fields BLANK if you do not perform the individual antibody testing or florescence.  Select florescence pattern result codes from the dropdown on the online form.

Instructions: AN Specimens

(766 Antinuclear Antibody (ANA) Latex Methods)

1. Remove the specimens from the refrigerator and allow them to come to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

4. Report ANA screen results as Antinuclear Antibody.

5. After testing is completed, store the vials in the freezer until you receive the evaluation of your results. If needed, the samples then can be thawed and re-tested.

Method Codes

Instructions: CR Specimens

(764 C-Reactive Protein)

(776 C-Reactive Protein – Add-On)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion. Test the sample in the same manner as you would test a patient sample.

3. Be sure to record your results in mg/dL. You must convert your results to these units to ensure you are graded correctly.

Instructions: AL Specimens (Allergens)

(770 Specific Allergen Testing)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample for the specific allergens listed.

Roman numerals refer to modified class scoring; arabic numerals refer to the conventional class scoring system.

4. If you do not perform a specific allergen, simply leave the result area blank. Do NOT report 0.

Instructions: AL Specimens (Total IgE)

(773 Diagnostic Allergy)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: HP Specimens

(780 H. pylori Antibody Detection)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by inversion.

2. If your testing kit uses capillary tubes for specimen draw: It is recommended that a specimen volume of 25µL be used for testing the HP samples. Use only 2 drops of developer during that step of the testing process.

   If your testing kit uses droppers/pipettes for specimen draw: Follow the testing instructions as indicated by your testing method.

       

METHOD CODES

Instructions: HIV Specimens

(791 HIV Markers)

(790 HIV Markers – Waived)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by

   inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: AS, IM & RF Specimens

(750 Immunology Module)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

   

Instructions: AS Specimens

(753 Anti-Streptolysin O – Add-On)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

Instructions: IMP Specimens

(784 Immunoproteins)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

Instructions: IM Specimens

(762 Infectious Mononucleosis)

(755 Infectious Mononucleosis – Waived)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by

   inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: MY Specimens

(781 Mycoplasma Antibody)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 10-15 minutes. Mix each vial by

   inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: OIV Specimens

(793 Oral Fluid HIV Antibodies)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: RF Specimens

(763 Rheumatoid Factor)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

Instructions: CR & RF Specimens

(751 Rheumatology Module)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

   

Instructions: RU Specimens

(771 Rubella)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by

   inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: SAB Specimens

(792 SARS-CoV-2 Serology)

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes. Mix each vial by

   inversion.

2. Test the sample in the same manner as you would test a patient sample.

Instructions: SY Specimens

(772 Syphilis Serology)

Syphilis Serology Result Codes

1. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

2. Mix each vial by inversion.

3. Test the sample in the same manner as you would test a patient sample.

4. Report the specific type of Syphilis testing performed on the appropriate line on the online form.

5. Select the appropriate qualitative or semi-quantitative result codes from the drop down as reported for patients.

Instructions: TOR Specimens

(752 ToRCH)

These are liquid serum samples ready for testing.

1. Remove specimens from the refrigerator and allow them to come to room temperature (20-25°C). This should take about 10-15 minutes.

2. Open the vial and remove a portion for testing. Immediately recap the vials and return to the refrigerator.

3. Test the sample in the same manner as you would test a patient sample.

Code results 10 for negative and 11 for positive.

For methods that do not differentiate between HSV I and HSV II, please enter results under HSV II results using result code "714" for positives.  For methods that are Ig non‐specific, report results as IgG using result code "715" for positives.

Instructions: VM Specimens

(775 Viral Markers)

CAUTION: These specimens contain dangerous pathogens and should be treated with extreme care. They should be handled as if capable of transmitting disease. Dispose of the specimens in the same manner as patient samples.

1. These are liquid samples that do not require reconstitution.

2. Remove the specimens from the refrigerator and warm to room temperature (20-25°C). This should take about 15-20 minutes.

3. Mix each vial by inversion. Invert the vials gently to avoid foaming or bubbling of the specimen.

4. Holding the vial in an upright position, gently tap the plastic screw cap to ensure that the entire sample is removed. Carefully remove the cap and remove a portion for testing. Immediately recap and return to the refrigerator when not in use.

5. Test the sample in the same manner as you would test a patient sample.

6. Complete all testing within 5 days of opening the vial. After all testing is completed, store the vials in the refrigerator until you receive the evaluation of your results. The sample can then be retested if necessary.